EMPIAR-10972 - Representative data from Near-native state imaging by cryo-soft-X-ray tomography reveals remodelling of cytoplasmic vesicles and mitochondria during HSV-1 infection [14 reconstructed volumes in MRC format]

EMPIAR-10972

Representative data from Near-native state imaging by cryo-soft-X-ray tomography reveals remodelling of cytoplasmic vesicles and mitochondria during HSV-1 infection [14 reconstructed volumes in MRC format]

Publication:

Near-native state imaging by cryo-soft-X-ray tomography reveals remodelling of cytoplasmic vesicles and mitochondria during HSV-1 infection

Nahas KLN, Connor VC, Harkiolaki M, Crump CM, Graham SC

bioRxiv (2021)

DOI: 10.1101/2021.10.11.463900

Related EMDB entry:

Deposited:

2021-11-17

Released:

2022-03-28

Last modified:

2022-03-28

Imageset size:

9.88 GB

Imageset DOI:

10.6019/EMPIAR-10972

Experimental metadata:

Download xml json

Contains:

reconstructed volumes

Image Sets

1. Representative cryoSXT datasets for studying changes to cellular compartments during HSV-1 infection of U2OS cells

Category:

reconstructed volumes

Image format:

MRC

No. of images or tilt series:

Image size:

(946, 946)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(100.0, 100.0)

Details:

Representative tomograms from the manuscript "Near-native state imaging by cryo-soft-X-ray tomography reveals remodelling of cytoplasmic vesicles and mitochondria during HSV-1 infection" can be found in the hsv_remodelling directory (5 GB).

DOI: 10.1101/2021.10.11.463900

hsv_perinuclear_viral_particle_hff-htert/
KLN_Dec2018_Box14_Grid2_D3F.SXT.FOV1.rec

early_stage_hsv_u2os/SXT/
KLN_July2020_Box1G2_B1F.SXT.FOV3.rec
KLN_July2020_Box1G2_D3A.SXT.FOV1.rec

hsv_cell_surface_viral_particles_hff-htert/SXT/
KLN_Feb2019_Feb2_Grid3_E3C.SXT.FOV1.rec

hsv_cytoplasmic_viral_particles_hff-htert/SXT/
KLN_Dec2018_Box12_Grid2_E4D.SXT.FOV2.rec

hsv_nuclear_capsids_hff-htert/SXT/
KLN_Feb2019_Feb2G1_D4F.SXT.FOV1.rec

late_stage_hsv_u2os/SXT/
BoxK1p3_Grid4_E5D.SXT.FOV2.rec
KLN_July2020_Box8G1_E2E.SXT.FOV2.rec

uninfected_u2os/SXT/
BoxK1p1_Grid2_A4B.SXT.FOV5.rec
KLN_July2020_Box2_G2_F5A.SXT.FOV1.rec

Tomograms from Supplementary figure 1 of the manuscript "Contour, a semi-automated segmentation and quantitation tool for cryo-soft-X-ray tomography" can be found in the contour_supplementary_figure directory (4.9GB).

DOI: 10.1101/2021.12.03.470962

HFF-hTERT/SXT/
KLN_Oct2020_Box8G2_E1C.SXT.FOV1.rec

HaCaT/SXT/
KLN_Jun2018_Box8G2_E5A.SXT.FOV1.rec

U2OS/SXT/
KLN_July2020_Box9G3_D5C.SXT.FOV1.rec

Vero/SXT/
NV_Box3G3_B3D.SXT.FOV1.rec

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