EMPIAR-10944 - Tutorial for high-throughput cryo-EM structure determination of amyloids: Dataset 3 [1259 multi-frame micrographs composed of 34 frames each in TIFF format]

EMPIAR-10944

Tutorial for high-throughput cryo-EM structure determination of amyloids: Dataset 3 [1259 multi-frame micrographs composed of 34 frames each in TIFF format]

Publication:

Assembly of recombinant tau into filaments identical to those of Alzheimer's disease and chronic traumatic encephalopathy

Lövestam S, Koh FA, van Knippenberg B, Kotecha A, Murzin AG, Goedert M, Scheres SHW

eLife 11 (2022)

PMID: 35244536

DOI: 10.7554/elife.76494

Related EMDB entry:

EMD-14054 EMD-14059 EMD-14056

Deposited:

2022-02-03

Released:

2022-02-22

Last modified:

2022-03-28

Imageset size:

839.82 GB

Imageset DOI:

10.6019/EMPIAR-10944

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame movies in Tiff: Dataset 3

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1259

Image size:

(None, None)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.824, 0.824)

Details:

Description: Movies were collected in EER format and converted into tiff using relion_convert_to_tiff grouping frames by 34 giving a dose of 0.98 e/Å^2. Please see processing pipeline for further details.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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