EMPIAR-10915 - CryoEM data of PLA2R at pH 6.2 with both 0 and 30 degree tilts. [multiple data sets in TIFF format]

EMPIAR-10915

CryoEM data of PLA2R at pH 6.2 with both 0 and 30 degree tilts. [multiple data sets in TIFF format]

Publication:

Structure of PLA2R reveals presentation of the dominant membranous nephropathy epitope and an immunogenic patch

Fresquet M, Lockhart-Cairns MP, Rhoden SJ, Jowitt TA, Briggs DC, Baldock C, Brenchley PE, Lennon R

Proceedings of the National Academy of Sciences of the United States of America 119 (2022)

PMID: 35858348

DOI: 10.1073/pnas.2202209119

Related EMDB entry:

EMD-14077

Deposited:

2020-06-17

Released:

2022-07-12

Last modified:

2022-10-10

Imageset size:

2.13 TB

Imageset DOI:

10.6019/EMPIAR-10915

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame images of PLA2R at pH 6.2 with a 30 degree tilt.

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2523

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.415, 0.415)

Details:

Images were collected at 30 degree tilt on a K3 in super res mode with sampling of 0.415 Å/pix. The gain image is in its own directory.
Titan Krios: 300 kV
Cs: 2.7
Frames: 40
Dose: 42.9 e/Å^2
Dose rate: 14.8 e.pix/sec

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Unaligned multi-frame images of PLA2R at pH 6.2 with 0 degree tilt (not used in publication)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3703

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.415, 0.415)

Details:

Images were collected at 0 degree tilt on a K3 in super res mode with sampling of 0.415 Å/pix. The gain image is in the main directory. This data was not used in the final publication.
Titan Krios: 300 kV
Cs: 2.7
Frames: 40
Dose: 42.9 e/Å^2
Dose rate: 14.8 e.pix/sec

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Quick Links

Talks and Tutorials

EMPIAR Quick tour

Statistics

Publications

Re-use case study

EMPIAR in the news

Claim entries to your ORCiD

COVID-19 Data Portal

EMDB

PDBe

BioImage Archive

EMNavigator@PDBj

PDBj

RCSB@PDBj

EMDataResoruce@RCSB,EMDB,NCMI

Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

See all citations

Protein Data Bank Japan News

[2024-11-27]

374 new PDB entries have been released on 2024-11-27.

[2024-11-01]

A new portal integrating genome variants and protein structures has released

[2024-10-30]

[wwPDB] Deprecation of FTP File Download Protocol on November 1, 2024

[2024-10-25]

PDBj luncheon seminar at APBJC 2024

[2024-10-17]

[wwPDB] The Nobel Prize in Chemistry 2024