EMPIAR-10906 - FIB-SEM of mouse optic nerve of an inducible conditional Mbp knock-out 16 weeks after induction [696 micrographs in TIFF format]

EMPIAR-10906

FIB-SEM of mouse optic nerve of an inducible conditional Mbp knock-out 16 weeks after induction [696 micrographs in TIFF format]

Publication:

White matter integrity in mice requires continuous myelin synthesis at the inner tongue

Meschkat M, Steyer AM, Weil MT, Kusch K, Jahn O, Piepkorn L, Agüi-Gonzalez P, Phan NTN, Ruhwedel T, Sadowski B, Rizzoli SO, Werner HB, Ehrenreich H, Nave KA, Möbius W

Nature communications 13 (2022)

PMID: 35246535

DOI: 10.1038/s41467-022-28720-y

Related EMDB entry:

Deposited:

2021-12-17

Released:

2022-01-12

Last modified:

2022-10-17

Imageset size:

15.88 GB

Imageset DOI:

10.6019/EMPIAR-10906

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. FIB-SEM data set of mouse optic nerve of an inducible conditional Mbp knock-out mouse 16 weeks after induction

Category:

micrographs - single frame

Image format:

TIFF

No. of images or tilt series:

696

Image size:

(6630, 3696)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(50.0, 50.0)

Details:

Optic nerve of a conditional and inducible Mbp knock-out mouse (Mbpfl/fl*PlpCreERT2+),16 weeks after tamoxifen-induction at the age of 8 weeks
Sample preparation: HPF and freeze substitution, embedding in EPON
FIB-SEM: SEM: 1.5 kV, ESB grid 450V, pixel size x/y 5 nm, continuous mill-and-acquire mode; FIB: 3 nA, z-step 50 nm
Postprocessing: alignment with Fiji/ImageJ TrackEM2, cropped, inverted and blurred (Gaussian blur, sigma 2) with Fiji/ImageJ

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