EMPIAR-10890 - Motion corrected micrographs - purified SINV/EEEV particles and recombinant anti-EEEV Fab (EEEV-33) [2111 micrographs in MRC format]

EMPIAR-10890

Motion corrected micrographs - purified SINV/EEEV particles and recombinant anti-EEEV Fab (EEEV-33) [2111 micrographs in MRC format]

Publication:

Human Antibodies Protect against Aerosolized Eastern Equine Encephalitis Virus Infection

Williamson LE, Gilliland T, Yadav PK, Binshtein E, Bombardi R, Kose N, Nargi RS, Sutton RE, Durie CL, Armstrong E, Carnahan RH, Walker LM, Kim AS, Fox JM, Diamond MS, Ohi MD, Klimstra WB, Crowe JE

Cell 183 (2020) 1884-1900.e23

PMID: 33301709

DOI: 10.1016/j.cell.2020.11.011

Related EMDB entry:

EMD-22223

Deposited:

2021-12-22

Released:

2022-01-12

Last modified:

2022-01-12

Imageset size:

111.82 GB

Imageset DOI:

10.6019/EMPIAR-10890

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Motion corrected micrographs Chimeric Eastern Equine Encephalitis Virus with Fab of EEEV-33 Antibody

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

2111

Image size:

(3838, 3710)

Pixel type:

SIGNED 32 BIT INTEGER

Pixel spacing:

(1.64, 1.64)

Details:

Images for SINV/EEEV:EEEV-33 Fab were collected on a Titan Krios electron microscope (Thermo Fisher Scientific) equipped with a K2 Summit Direct Electron Detector operated at 300 kV and having a nominal pixel size of 1.64 Å per pixel. Micrographs were acquired automatically using Leginon software. The total exposure time was 10 s and frames were recorded every 0.2 s. Defocus values ranged from 1.0 to 2.5 μm. The total accumulated does was ~50 e−/Å2. Deposited micrographs were motion corrected using MotionCor2.

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