EMPIAR-10825 - Cryo electron microscopy of ex-vivo murine SAA amyloid fibrils [1429 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-10825

Cryo electron microscopy of ex-vivo murine SAA amyloid fibrils [1429 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Cryo-EM fibril structures from systemic AA amyloidosis reveal the species complementarity of pathological amyloids

Liberta F, Loerch S, Rennegarbe M, Schierhorn A, Westermark P, Westermark GT, Hazenberg BPC, Grigorieff N, Fändrich M, Schmidt M

Nature communications 10 (2019)

PMID: 30846696

DOI: 10.1038/s41467-019-09033-z

Related EMDB entry:

EMD-8910

Deposited:

2021-06-10

Released:

2021-11-02

Last modified:

2021-11-02

Imageset size:

533.91 GB

Imageset DOI:

10.6019/EMPIAR-10825

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multiframe micrographs of ex-vivo murine SAA1

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1429

Image size:

(7676, 7420)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.675, 0.675)

Details:

Data collection with a Titan Krios EM (300 kV) using a K2 Summit camera, 20 eV slit. Defocus range -1.3 to -5.5, exposure time 10.2 and 8 seconds, dose rate 1.967 e/A^2/sec (set 1), 2.521 e/A^2/sec (set 2), total dose 20 e/A^2.
CS = 2.7 mm, HT = 300 kV, AmpCnst = 0.07.
In addition, thumbnails, power-spectra (data/thumbnails) and the gain reference (data/gain_reference) of ex-vivo murine SAA1 micrographs can be found within this entry.

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