EMPIAR-10795 - A microtubule RELION-based pipeline for cryo-EM image processing [1128 multi-frame micrographs composed of 64 frames each in MRC format]

EMPIAR-10795

A microtubule RELION-based pipeline for cryo-EM image processing [1128 multi-frame micrographs composed of 64 frames each in MRC format]

Publication:

A microtubule RELION-based pipeline for cryo-EM image processing

Cook AD, Manka SW, Wang S, Moores CA, Atherton J

Journal of Structural Biology (2019)

PMID: 31610239

DOI: 10.1016/j.jsb.2019.10.004

Related EMDB entry:

EMD-4643

Deposited:

2019-07-24

Released:

2021-09-24

Last modified:

2022-02-04

Imageset size:

1.65 TB

Imageset DOI:

10.6019/EMPIAR-10795

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Movies of microtubules decorated with CAMSAP1-CKK domain

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

1128

Image size:

(3710, 3838)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(None, None)

Details:

64 frames over 16 seconds.
42 electron per squared Angstrom total dose over the whole movie.
Pixel size 1.39 Angstrom/Pixel.
Camera and microscope: K2 summit in counting mode on a Technai Polara at 300kV.

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Citations

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Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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