EMPIAR-10792 - Structural mechanism of GTPase-powered ribosome-tRNA movement [multiple data sets in MRC and MRCS formats]

EMPIAR-10792

Structural mechanism of GTPase-powered ribosome-tRNA movement [multiple data sets in MRC and MRCS formats]

Publication:

Structural mechanism of GTPase-powered ribosome-tRNA movement

Petrychenko V, Peng BZ, de A P Schwarzer AC, Peske F, Rodnina MV, Fischer N

Nature communications 12 (2021)

PMID: 34635670

DOI: 10.1038/s41467-021-26133-x

Related EMDB entry:

EMD-13460 EMD-13462 EMD-13458 EMD-13461 EMD-13464 EMD-13463 EMD-13465 EMD-13459

Deposited:

2021-08-23

Released:

2021-11-10

Last modified:

2021-11-10

Imageset size:

987.53 GB

Imageset DOI:

10.6019/EMPIAR-10792

Experimental metadata:

Download xml json

Contains:

micrographs - single frame
picked particles - single frame - processed

Image Sets

1. Motion-corrected, dose-weighted micrographs

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

9300

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.16, 1.16)

Details:

Note: Micrographs were obtained from movie-stacks of 20 frames à 1.5 e/A^2 by global motion-correction and dose-weighting using MotionCor2 in Relion.
- Micrographs .star-file: "corrected_micrographs.star"

Files:

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2. Shiny particles of non-rotated ribosome particles (C state), obtained by Bayesian polishing in Relion 3.1

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

1176423

Image size:

(288, 288)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.16, 1.16)

Details:

Shiny particles .star-file & 3D mask for final 3D refinement of C state (EMD-13458): "shiny_Cstate.star" & "mask_Cstate.mrc"

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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3. Shiny particles of rotated ribosome particles, obtained by Bayesian polishing in Relion 3.1

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

103222

Image size:

(288, 288)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.16, 1.16)

Details:

Star-files of shiny particles & 3D masks used for final 3D refinements:
- H1 (EMD-13459): "shiny_H1.star" & "mask_H1.mrc"
- H2 (EMD-13460): "shiny_H2.star" & "mask_H2.mrc"
- H1-EF-G-GDP-Pi (EMD-13461): "shiny_H1-EF-G-GDP-Pi.star" & "mask_H1-EF-G-GDP-Pi.mrc"
- H2-EF-G-GDP-Pi (EMD-13462): "shiny_H2-EF-G-GDP-Pi.star" & "mask_H2-EF-G-GDP-Pi.mrc"
- H1-EF-G-GDP (EMD-13463): "shiny_H1-EF-G-GDP.star" & "mask_H1-EF-G-GDP.mrc"
- CHI1-EF-G-GDP (EMD-13464): "shiny_CHI1-EF-G-GDP.star" & "mask_CHI1-EF-G-GDP.mrc"
- CHI2-EF-G-GDP (EMD-13465): "shiny_CHI2-EF-G-GDP.star" & "mask_CHI2-EF-G-GDP.mrc"
Star-files of shiny particles for substates 1-3 of EF-G domain 4 in H1-EF-G-GDP-Pi state (EMD-13461):
- "shiny_H1-EF-G-GDP-Pi_D4_substate1.star"
- "shiny_H1-EF-G-GDP-Pi_D4_substate2.star"
- "shiny_H1-EF-G-GDP-Pi_D4_substate3.star"

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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