EMPIAR-10767 - Single particle cryo-EM dataset of sarkosyl-insoluble fraction from the temporal cortex of an individual with limbic-predominant neuronal inclusion body 4R tauopathy [10615 multi-frame micrographs composed of 1351 frames each in EER format]

EMPIAR-10767

Single particle cryo-EM dataset of sarkosyl-insoluble fraction from the temporal cortex of an individual with limbic-predominant neuronal inclusion body 4R tauopathy [10615 multi-frame micrographs composed of 1351 frames each in EER format]

Publication:

Structure-based classification of tauopathies

Shi Y, Zhang W, Yang Y, Murzin AG, Falcon B, Kotecha A, van Beers M, Tarutani A, Kametani F, Garringer HJ, Vidal R, Hallinan GI, Lashley T, Saito Y, Murayama S, Yoshida M, Tanaka H, Kakita A, Ikeuchi T, Robinson AC, Mann DMA, Kovacs GG, Revesz T, Ghetti B, Hasegawa M, Goedert M, Scheres SHW

Nature 598 (2021) 359-363

PMID: 34588692

DOI: 10.1038/s41586-021-03911-7

Related EMDB entry:

EMD-13224 EMD-13223 EMD-13225

Deposited:

2021-07-17

Released:

2021-09-17

Last modified:

2021-10-19

Imageset size:

5.46 TB

Imageset DOI:

10.6019/EMPIAR-10767

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame movies

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

10615

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.727, 0.727)

Details:

Movies were acquired on a Titan Krios G4 microscope operated at 300 kV, which was equipped with a cold field-emission gun, a Falcon-IV detector, and a Selectrix X energy filter using a slit width of 10 eV. The total dose per movie is ~50 e/Å^2. Please use Movies/EER_GainReference.mrc as a gain reference without any rotation or flipping.

data/GPT/Segments_coordinates contains segment coordinates picked by crYOLO.

data/GPT/data.star contains STAR files with the metadata of the final refined segments.

data/GPT/XMLs contains EPU metadata files.

Cfeg flash time stamp:
1 | 2020-09-05 14:34:25
2 | 2020-09-05 15:19:16
3 | 2020-09-05 17:16:53
4 | 2020-09-06 00:42:08
5 | 2020-09-06 11:53:49
6 | 2020-09-06 21:50:36

Nitrogen fill time stamp:
1 | 2020-09-05 15:16:44
2 | 2020-09-05 22:38:55
3 | 2020-09-06 05:21:31
4 | 2020-09-06 12:13:43
5 | 2020-09-06 19:04:52
6 | 2020-09-07 01:48:23

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Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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