EMPIAR-10767
Single particle cryo-EM dataset of sarkosyl-insoluble fraction from the temporal cortex of an individual with limbic-predominant neuronal inclusion body 4R tauopathy [10615 multi-frame micrographs composed of 1351 frames each in EER format]
Publication:

Structure-based classification of tauopathies

Shi Y, Zhang W, Yang Y, Murzin AG, Falcon B, Kotecha A, van Beers M, Tarutani A, Kametani F, Garringer HJ, Vidal R, Hallinan GI, Lashley T, Saito Y, Murayama S, Yoshida M, Tanaka H, Kakita A, Ikeuchi T, Robinson AC, Mann DMA, Kovacs GG, Revesz T, Ghetti B, Hasegawa M, Goedert M, Scheres SHW

Nature 598 (2021) 359-363

PMID: 34588692

Related EMDB entry:
Deposited:
2021-07-17
Released:
2021-09-17
Last modified:
2021-10-19
Imageset size:
5.46 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned multi-frame movies
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
10615
Image size:
(4096, 4096)
Pixel type:
BIT
Pixel spacing:
(0.727, 0.727)
Details:
Movies were acquired on a Titan Krios G4 microscope operated at 300 kV, which was equipped with a cold field-emission gun, a Falcon-IV detector, and a Selectrix X energy filter using a slit width of 10 eV. The total dose per movie is ~50 e/Å^2. Please use Movies/EER_GainReference.mrc as a gain reference without any rotation or flipping.

data/GPT/Segments_coordinates contains segment coordinates picked by crYOLO.

data/GPT/data.star contains STAR files with the metadata of the final refined segments.

data/GPT/XMLs contains EPU metadata files.

Cfeg flash time stamp:
1 | 2020-09-05 14:34:25
2 | 2020-09-05 15:19:16
3 | 2020-09-05 17:16:53
4 | 2020-09-06 00:42:08
5 | 2020-09-06 11:53:49
6 | 2020-09-06 21:50:36

Nitrogen fill time stamp:
1 | 2020-09-05 15:16:44
2 | 2020-09-05 22:38:55
3 | 2020-09-06 05:21:31
4 | 2020-09-06 12:13:43
5 | 2020-09-06 19:04:52
6 | 2020-09-07 01:48:23
Files:
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