EMPIAR-10765 - Single particle cryo-EM dataset of sarkosyl-insoluble fraction from the frontal cortex of an individual with progressive supranuclear palsy [6601 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-10765

Single particle cryo-EM dataset of sarkosyl-insoluble fraction from the frontal cortex of an individual with progressive supranuclear palsy [6601 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Structure-based classification of tauopathies

Shi Y, Zhang W, Yang Y, Murzin AG, Falcon B, Kotecha A, van Beers M, Tarutani A, Kametani F, Garringer HJ, Vidal R, Hallinan GI, Lashley T, Saito Y, Murayama S, Yoshida M, Tanaka H, Kakita A, Ikeuchi T, Robinson AC, Mann DMA, Kovacs GG, Revesz T, Ghetti B, Hasegawa M, Goedert M, Scheres SHW

Nature 598 (2021) 359-363

PMID: 34588692

DOI: 10.1038/s41586-021-03911-7

Related EMDB entry:

EMD-13218

Deposited:

2021-07-07

Released:

2021-09-17

Last modified:

2021-10-19

Imageset size:

967.35 GB

Imageset DOI:

10.6019/EMPIAR-10765

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6601

Image size:

(3838, 3710)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.15, 1.15)

Details:

The files were written as gain-unnormalised integer MRC files by EPU and then converted to TIFF files by mrc2tif in the IMOD suite. During this process, the pixel size in the header was lost. The real pixel size is 1.15 A/px. The dose rate is 1.0155 e/A2/frame. Please use Movies/gain.mrc as a gain reference without any rotation or flipping.

data/PSP/Start_end_coordinates contains start-end coordinates of the manually picked filaments.

data/PSP/Data.star contains STAR file with the metadata of the final refined segments.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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