EMPIAR-10633 - Cryo-electron tomography of the metazoan membrane-assembled retromer:SNX3 coat containing Wls cargo motif [multiple data sets in TIFF and MRC formats]

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EMPIAR-10633

Cryo-electron tomography of the metazoan membrane-assembled retromer:SNX3 coat containing Wls cargo motif [multiple data sets in TIFF and MRC formats]

Publication:

Architecture and mechanism of metazoan retromer:SNX3 tubular coat assembly

Leneva N, Kovtun O, Morado DR, Briggs JAG, Owen DJ

Science advances 7 (2021)

PMID: 33762348

DOI: 10.1126/sciadv.abf8598

Related EMDB entry:

EMD-12222 EMD-12221 EMD-12220

Deposited:

2021-01-18

Released:

2021-04-14

Last modified:

2021-06-30

Imageset size:

764.90 GB

Imageset DOI:

10.6019/EMPIAR-10633

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
tilt series

Image Sets

1. Raw image frames for the metazoan retromer:SNX3 coat assembled on the Wls cargo-containing membranes

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4844

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.8505, 0.8505)

Details:

Unaligned superresolution frames not corrected for detector’s gain and defects. Ten frames were saved per each tilt image. The exposure dose per tilt image was 3.0 e/Å^2. Calibrated physical pixel size 1.701 Å. Movies are named as ts_[tilt stack ID]_[sequntial movie number]_[tilt angle].tif.
For gain reference, use data/correction/SuperRef_ts_01_14.dm4 for tilts ts_01 to 14, and data/correction/SuperRef_ts_16_126.dm4 for tilts ts_16 to 126. The defect file data/correction/Krios3_K3_SerialEM_defects.txt to be applied to all tilt series.

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2. Corrected, aligned and order-sorted tilt series for the metazoan retromer:SNX3 coat assembled on the Wls cargo-containing membranes

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

113

Image size:

(5760, 4092)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.701, 1.701)

Details:

Corrected, aligned and order-sorted tilt series. The tilt-series were generated from raw frames (data/rawframes) by applying gain reference and defect corrections, motion correction and Fourier cropping to physical pixel size. No dose-filtering was applied. Defocus estimation was performed using IMOD ctfplotter on non-dose filtered aligned stacks (data/aligned_stacks). ETOMO generated tilt alignment using 10 nm gold fiducials tracking.

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3. Corrected, aligned, dose-filtered and order-sorted tilt series for the metazoan retromer:SNX3 coat assembled on the Wls cargo-containing membranes

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

113

Image size:

(5760, 4092)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.701, 1.701)

Details:

Dose-filtered aligned stacks.

Files:

This entry cannot be downloaded from PDBj.

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We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

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