EMPIAR-10621 - Lysosomes as Fiducials for SXT and SIM Correlation [166 tilt series in TIFF format]

EMPIAR-10621

Lysosomes as Fiducials for SXT and SIM Correlation [166 tilt series in TIFF format]

Publication:

Sample preparation strategies for efficient correlation of 3D imaging data at cryogenic temperatures: the journey towards multimodal bioimaging

Okolo CA, Kounatidis I, Groen J, Nahas KL, Balint S, Fish T, Koronfel MA, López-Cortajarena A, Dobbie I, Pereiro E, Harkiolaki M

Nature Protocols (2021)

Related EMDB entry:

Deposited:

2021-01-18

Released:

2021-09-01

Last modified:

2021-09-01

Imageset size:

170.30 MB

Imageset DOI:

10.6019/EMPIAR-10621

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. SXT using Lysosome and non-fluorescent Au as fiducials

Category:

tilt series

Image format:

TIFF

No. of images or tilt series:

166

Image size:

(946, 806)

Pixel type:

SIGNED 32 BIT INTEGER

Pixel spacing:

(160.0, 160.0)

Details:

---

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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