EMPIAR-10583 - Single-particle Cryo-EM of the Thermococcus gammatolerans McrBC complex [2178 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-10583

Single-particle Cryo-EM of the Thermococcus gammatolerans McrBC complex [2178 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Structural asymmetry governs the assembly and GTPase activity of McrBC restriction complexes

Niu Y, Suzuki H, Hosford CJ, Walz T, Chappie JS

Nature communications 11 (2020)

PMID: 33219217

DOI: 10.1038/s41467-020-19735-4

Related EMDB entry:

EMD-20866

Deposited:

2020-11-22

Released:

2020-12-04

Last modified:

2020-12-04

Imageset size:

948.41 GB

Imageset DOI:

10.6019/EMPIAR-10583

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of the Thermococcus gammatolerans McrBC complex

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2178

Image size:

(7420, 7676)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.5, 0.5)

Details:

Gain reference is included as reference.mrc in the same folder with the movie files. The gain reference must be rotated 90 degrees and flipped upside down. Dose per frame: 2 e/A^2

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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