EMPIAR-10533 - SARS-CoV-2 infection in human adult lung alveolar stem cells [multiple data sets in TIFF format]

EMPIAR-10533

SARS-CoV-2 infection in human adult lung alveolar stem cells [multiple data sets in TIFF format]

Publication:

Three-Dimensional Human Alveolar Stem Cell Culture Models Reveal Infection Response to SARS-CoV-2

Youk J, Kim T, Evans KV, Jeong YI, Hur Y, Hong SP, Kim JH, Yi K, Kim SY, Na KJ, Bleazard T, Kim HM, Fellows M, Mahbubani KT, Saeb-Parsy K, Kim SY, Kim YT, Koh GY, Choi BS, Ju YS, Lee JH

Cell stem cell 27 (2020) 905-919.e10

PMID: 33142113

DOI: 10.1016/j.stem.2020.10.004

Related EMDB entry:

Deposited:

2020-10-09

Released:

2020-11-18

Last modified:

2020-11-18

Imageset size:

20.79 GB

Imageset DOI:

10.6019/EMPIAR-10533

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Infected h3AC traditional transmission electron microscopy at 2 day post infection

Category:

micrographs - single frame

Image format:

TIFF

No. of images or tilt series:

251

Image size:

(4096, 4224)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(None, None)

Details:

Infected h3AC 2 day post infection transmission electron microscopy.
Total 5 h3ACs were taken and their pictures were stored under the infected h3AC as different numbers.
For example, infected.h3AC.Figure.4.A means one of the infected h3AC and the pictures were used to make Figure 4.A of the research paper.

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2. Infected h3BC traditional transmission electron microscopy at 2 day post infection

Category:

micrographs - single frame

Image format:

TIFF

No. of images or tilt series:

Image size:

(4096, 4224)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(None, None)

Details:

Infected h3BC were taken at 2 day post infection.

Only one h3BC was taken as picture.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Uninfected h3ACs 2D traditional electron microscopy

Category:

micrographs - single frame

Image format:

TIFF

No. of images or tilt series:

326

Image size:

(4096, 4224)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(None, None)

Details:

Control h3ACs were taken at P1-2 from donors.
Total number of h3ACs were 20. The subdirectory was divided into 3 folders with the name of the date of picture taken.
0724(2020) 5 h3ACs were taken and 55 pictures existed
0811(2020) 8 h3ACs were taken and 122 pictures existed
0812(2020) 7 h3ACs were taken and 149 pictures existed
For 0811 and 0812 folders, there is also an .emi and .ser file which is the original file of the TEM.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

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Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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