EMPIAR-10471 - CryoEM structure of human CMG bound to ATPgammaS and DNA [3694 multi-frame micrographs composed of 40 frames each in MRC format]

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EMPIAR-10471

CryoEM structure of human CMG bound to ATPgammaS and DNA [3694 multi-frame micrographs composed of 40 frames each in MRC format]

Publication:

CryoEM structures of human CMG-ATPγS-DNA and CMG-AND-1 complexes

Rzechorzek NJ, Hardwick SW, Jatikusumo VA, Chirgadze DY, Pellegrini L

Nucleic acids research 48 (2020) 6980-6995

PMID: 32453425

DOI: 10.1093/nar/gkaa429

Related EMDB entry:

EMD-10619 EMD-10620

Deposited:

2020-04-30

Released:

2020-08-18

Last modified:

2020-08-18

Imageset size:

8.41 TB

Imageset DOI:

10.6019/EMPIAR-10471

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multiframe micrographs of human CMG bound ATPgammaS and DNA

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

3694

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.07, 1.07)

Details:

Corrected, unaligned counting mode frame stacks (40 frames) of the human CMG helicase bound to DNA and ATP-gamma-S, collected on a Titan Krios with a Gatan K2 Summit direct electron detector.

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

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CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

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Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

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Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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