EMPIAR-10444
Cryo-EM structures of calcium homeostasis modulator (CALHM) channels [multiple data sets in TIFF format]
Publication:

Cryo-EM structures of calcium homeostasis modulator channels in diverse oligomeric assemblies

Demura K, Kusakizako T, Shihoya W, Hiraizumi M, Nomura K, Shimada H, Yamashita K, Nishizawa T, Taruno A, Nureki O

Science Advances 6 (2020)

Deposited:
2020-06-10
Released:
2020-07-30
Last modified:
2020-07-30
Imageset size:
7.43 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned movies for OlCALHM1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
3075
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.83, 0.83)
Details:
Gain reference is provided, which need to be flipped ('Flip upside down (1)' in RELION).
Files:
Loading...
2. Unaligned movies for HsCALHM2
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
2110
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(0.8346, 0.8346)
Details:
Unaligned, gain corrected movies from Falcon 3 in the counting mode. The MRC files from EPU were converted to the TIFF format by mrc2tif in IMOD. During this process, the pixel size in the image header became 1.0. Note that we started processing with the nominal pixel size of 0.861 Å and finally changed the value in PostProcess so that the pixels had the calibrated size of 0.8346 Å.
Files:
Loading...
3. Unaligned movies for CeCLHM-1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
2906
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(0.8346, 0.8346)
Details:
Unaligned, gain corrected movies from Falcon 3 in the counting mode. The MRC files from EPU were converted to the TIFF format by mrc2tif in IMOD. During this process, the pixel size in the image header became 1.0. Note that we started processing with the nominal pixel size of 0.861 Å and finally changed the value in PostProcess so that the pixels had the calibrated size of 0.8346 Å.
Files:
Loading...
4. Unaligned movies for OlCALHM1-HsCALHM2 chimera
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
2808
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.83, 0.83)
Details:
Gain reference is provided, which need to be flipped ('Flip upside down (1)' in RELION).
Files:
Loading...
Kim K, Li H, Clarke OB. (2025)
Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)
Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)
Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)
Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)
Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)
Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)
Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)
Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)
Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)