EMPIAR-10443
Mechanism of Ribosome Rescue by Alternative Release Factor B [multiple data sets in MRC format]
Publication:

Mechanism of Ribosome Rescue by Alternative Release Factor B

Chan K-H, Petrychenko V, Mueller C, Maracci C, Holtkamp W, Wilson DN, Fischer N, Rodnina MV

Nature Communications 11 (2020)

PMID: 27906160

Related EMDB entry:
Deposited:
2020-06-11
Released:
2020-08-18
Last modified:
2020-08-18
Imageset size:
788.20 GB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - single frame
1. Motion-corrected, dose-weighted micrographs & polished particles of P+9 E. coli ribosome-ArfB complex
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
4750
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.16, 1.16)
Details:
Note: Micrographs were obtained from movie-stacks of 40 frames à 1.25 e/A^2 by global motion-correction and dose-weighting using MotionCor2 in Relion, "shiny" particles were obtained by Baysian polishing in Relion 3.0
- Micrographs .star-file: "micrographs_ctf.star"
- Particles .star-file for all particles: "particles_all.star"
- Particles .star-file for particles of the P+9 stalled ribosome complex (EMD-10905): "particles_P+9_stalled_complex.star"
- Particles .star-file for particles of P+9 ArfB-ribosome complex in the post-hydrolysis state (EMD-10906): "particles_P+9 post-hydrolysis state.star"
- Particles .star-file for particles of P+9 ArfB-ribosome complex with P/E hybrid tRNA in the post-hydrolysis state (EMD-10907): "particles_P+9 post-hydrolysis state with PE hybrid tRNA.star"
Files:
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2. Motion-corrected, dose-weighted micrographs & extracted particles of P+0 E. coli ribosome-ArfB complex
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
3633
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.072, 1.072)
Details:
Note: Micrographs were obtained from movie-stacks of 10 frames à 2.5 e/A^2 by global motion-correction and dose-weighting using MotionCor2 in Relion
- Micrographs .star-file: "micrographs_ctf.star"
- Particles .star-file for particles of P+0 ArfB-ribosome complex in the post-hydrolysis state (EMD-10908): "particles_P+0 post-hydrolysis state.star"
Files:
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