EMPIAR-10439 - SARS-CoV-2 ORF3a dimer in an MSP1E3D1 lipid nanodisc [6309 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-10439

SARS-CoV-2 ORF3a dimer in an MSP1E3D1 lipid nanodisc [6309 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

Cryo-EM structure of the SARS-CoV-2 3a ion channel in lipid nanodiscs

Kern DM, Sorum B, Mali SS, Hoel CM, Sridharan S, Remis JP, Toso DB, Kotecha A, Bautista DM, Brohawn SG

bioRxiv (2020)

DOI: 10.1101/2020.06.17.156554

Related EMDB entry:

EMD-22136

Deposited:

2020-06-03

Released:

2020-06-19

Last modified:

2022-01-31

Imageset size:

4.35 TB

Imageset DOI:

10.6019/EMPIAR-10439

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame movies of SARS-CoV-2 ORF3a dimer in an MSP1E3D1 lipid nanodisc

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6309

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.5685, 0.5685)

Details:

Gain reference is in frames folder. Dose per frame for movies in "frames" is 1.00044 e/A2 and dose per frame for movies in "frames2" folder is 1.0135 e/A2. The final polished particles are in "3a-for-empiar". Movies were taken on a Talos Arctica with K3 camera.

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

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