EMPIAR-10406 - Motion-corrected micrographs and extracted particle images of the 70S ribosome from the human pathogen Acinetobacter baumannii in complex with amikacin [multiple data sets in MRC and MRCS formats]

EMPIAR-10406

Motion-corrected micrographs and extracted particle images of the 70S ribosome from the human pathogen Acinetobacter baumannii in complex with amikacin [multiple data sets in MRC and MRCS formats]

Publication:

Structure of the 70S Ribosome from the Human Pathogen Acinetobacter baumannii in Complex with Clinically Relevant Antibiotics

Nicholson D, Edwards TA, O'Neill AJ, Ranson NA

Structure (London, England : 1993) 28 (2020) 1087-1100.e3

PMID: 32857965

DOI: 10.1016/j.str.2020.08.004

Related EMDB entry:

EMD-10809 EMD-10869 EMD-10892

Deposited:

2020-05-05

Released:

2020-11-24

Last modified:

2022-02-08

Imageset size:

190.78 GB

Imageset DOI:

10.6019/EMPIAR-10406

Experimental metadata:

Download xml json

Contains:

micrographs - single frame
picked particles - multiframe - processed

Image Sets

1. Motion-corrected micrographs of the 70S ribosome from the human pathogen Acinetobacter baumannii in complex with amikacin

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

2717

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.07, 1.07)

Details:

Drift-corrected and dose-corrected averages of movies collected at an accumulated dose of 58 e-/A^2 across 50 frames over a 10 s exposure, recorded by a Gatan K2 Summit detector. Motion correction was performed using MotionCor2 within RELION 3.0, by using all frames, applying a B-factor of 150, and using 5 patches in both the X and Y directions. Coordinates corresponding to extracted particle images are in the particles.star file.

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2. Extracted particle images of the 70S ribosome from the human pathogen Acinetobacter baumannii in complex with amikacin

Category:

picked particles - multiframe - processed

Image format:

MRCS

No. of images or tilt series:

554

Image size:

(416, 416)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.07, 1.07)

Details:

Particles were extracted from motion-corrected, CTF-estimated micrographs into 416 square pixel boxes with re-centring of refined coordinates. Images of particles extracted from each micrograph are stacked into separate mrcs files. Coordinates and further metadata corresponding to extracted particle images are in the particle.star file.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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