EMPIAR-10385 - Cryo-EM structure of Lumazine Synthase [2874 multi-frame micrographs composed of 39 frames each in MRC format]

EMPIAR-10385

Cryo-EM structure of Lumazine Synthase [2874 multi-frame micrographs composed of 39 frames each in MRC format]

Publication:

Cryo-electron microscopy: an introduction to the technique, and considerations when working to establish a national facility

Bhella D

Biophysical reviews 11 (2019) 515-519

PMID: 31359340

DOI: 10.1007/s12551-019-00571-w

Related EMDB entry:

EMD-10736

Deposited:

2020-03-11

Released:

2020-04-09

Last modified:

2022-01-31

Imageset size:

6.84 TB

Imageset DOI:

10.6019/EMPIAR-10385

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Frame aligned multi-frame movies of Lumazine Synthase imaged on a CryoARM300 and DE64

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

2874

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.597, 0.597)

Details:

Lumazine Synthase was expressed in E.coli and purified by immobilised metal affinity chromatography. Particles were frozen on C-flat R1.2/1.3 grids. Imaging was performed at 200 kX magnification on a JEOL CryoARM 300 equipped with a Direct Electron DE64 detector. Images were collected using SerialEM with image-beam shift to record two shots per hole. The energy-filter slit was not inserted. Images were collected in electron counting mode with two times binning.
To correct for some residual fixed-pattern in the images, a post-collection gain-reference estimate was calculated using Relion, and applied using MotionCor2 with whole-frame motion correction.

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