EMPIAR-10383 - CryoEM structure of human LRRC8D [3399 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-10383

CryoEM structure of human LRRC8D [3399 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Cryo-EM structure of the volume-regulated anion channel LRRC8D isoform identifies features important for substrate permeation

Nakamura R, Numata T, Kasuya G, Yokoyama T, Nishizawa T, Kusakizako T, Kato T, Hagino T, Dohmae N, Inoue M, Watanabe K, Ichijo H, Kikkawa M, Shirouzu M, Jentsch TJ, Ishitani R, Okada Y, Nureki O

Communications biology 3 (2020)

PMID: 32415200

DOI: 10.1038/s42003-020-0951-z

Related EMDB entry:

EMD-30029

Deposited:

2020-03-05

Released:

2021-11-09

Last modified:

2021-11-09

Imageset size:

1.71 TB

Imageset DOI:

10.6019/EMPIAR-10383

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned movies of human LRRC8D isoform

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3399

Image size:

(7420, 7676)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.49, 1.49)

Details:

Movies were collected on Talos Arctica at 0.745 A / super-resolution pixel (that is, 1.49 A / physical pixel). Gain references must be rotated and flipped. In MotionCor2, this is "-RotGain 1 -FlipGain 2". Seven datasets were combined to yield the structure.
Dose rate (shown on SerialEM) of Datasets 0 and 1 are 1.245 e/A2/frame

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