EMPIAR-10359
Cryo-EM of PAC1 receptor bound to PACAP38 and Gs protein [7649 multi-frame micrographs composed of 62 frames each in TIFF format]
Publication:

Toward a Structural Understanding of Class B GPCR Peptide Binding and Activation

Liang YL, Belousoff MJ, Zhao P, Koole C, Fletcher MM, Truong TT, Julita V, Christopoulos G, Xu HE, Zhang Y, Khoshouei M, Christopoulos A, Danev R, Sexton PM, Wootten D

Molecular cell 77 (2020) 656-668.e5

PMID: 32004469

Related EMDB entry:
Deposited:
2020-02-03
Released:
2020-11-23
Last modified:
2020-11-23
Imageset size:
4.78 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned multi-frame gain-normalized movies in LZW compressed TIFF format
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
7649
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.83, 0.83)
Details:
- Approximately half of the data were collected with the Volta phase plate (VPP). Such movies are in the "VPP" subfolder and contain "VPP" in their filename.
- The movies were collected with a 9-hole beam-image shift acquisition pattern. The filename contains a four digit number in the range 0000 to 0008 corresponding to the image-shift acquisition position.
- The image-shift induced beam-tilt was not compensated optically during acquisition. For best results, perform per-image-shift-position beam-tilt measurement and correction with CTF refinement.
- The movies were recorded with Gatan K3 camera in counting (hardware bin by 2) mode and saved as gain-normalized LZW compressed TIFF files by SerialEM.
Files:
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