EMPIAR-10342 - Cryo-EM structure of the formoterol-bound turkey beta1 adrenoceptor coupled to beta-arrestin-1 with bound Fab30 in lipid nanodisc [multiple data sets in TIFF and MRCS formats]

EMPIAR-10342

Cryo-EM structure of the formoterol-bound turkey beta1 adrenoceptor coupled to beta-arrestin-1 with bound Fab30 in lipid nanodisc [multiple data sets in TIFF and MRCS formats]

Publication:

Molecular basis of β-arrestin coupling to formoterol-bound β1-adrenoceptor

Lee Y, Warne T, Nehmé R, Pandey S, Dwivedi-Agnihotri H, Chaturvedi M, Edwards PC, García-Nafría J, Leslie AGW, Shukla AK, Tate CG

Nature (2020)

PMID: 32555462

DOI: 10.1038/s41586-020-2419-1

Related EMDB entry:

EMD-10515

Deposited:

2019-11-29

Released:

2020-07-28

Last modified:

2021-10-15

Imageset size:

4.00 TB

Imageset DOI:

10.6019/EMPIAR-10342

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
picked particles - multiframe - processed
picked particles - multiframe - unprocessed

Image Sets

1. Unaligned multi-frame micrographs

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

9026

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.1, 1.1)

Details:

data/Movies/190830 contains unaligned movies related to collection "LMB Krios2-1". The "_hn_"-portion of the filename, where n=1-9, denotes the hole position within the 3x3 image-shift acquisition matrix. Two exposures per hole are differentiated by the time-stamp portion of the filename (~20 seconds apart). Micrographs were collected with a nominal stage-tilt of 30deg. The files were written as gain-unnormalised integer TIFF files by serialEM. The real pixel size is 1.1 Å/pix. The dose rate is 1.02 e/Å^2/frame. Please use data/Movies/GainRef/190830_gainref.mrc as gain reference without any rotation or flipping. The associated K2 defects file is data/Movies/Defect/190830_defects.txt.

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2. Unaligned multi-frame micrographs

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6808

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.1, 1.1)

Details:

data/Movies/190907 contains unaligned movies related to collection "LMB Krios2-2". The "_hn_"-portion of the filename, where n=1-9, denotes the hole position within the 3x3 image-shift acquisition matrix. Two exposures per hole are differentiated by the time-stamp portion of the filename (~20 seconds apart). Micrographs were collected with a nominal stage-tilt of 30deg. The files were written as gain-unnormalised integer TIFF files by serialEM. The real pixel size is 1.1 Å/pix. The dose rate is 0.98 e/Å^2/frame. Please use data/Movies/GainRef/190907_gainref.mrc as gain reference without any rotation or flipping. The associated K2 defects file is data/Movies/Defect/190907_defects.txt.

Files:

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3. Unaligned multi-frame micrographs

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2747

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.047, 1.047)

Details:

data/Movies/190911 contains unaligned movies related to collection "DLS eBIC Krios1". Two exposures per hole are differentiated by the two fields following "_Data_" in the filename. Micrographs were collected with a nominal stage-tilt of 30deg. The files were written as gain-unnormalised integer MRC files by EPU and then converted to TIFF files by mrc2tif in the IMOD suite. During this process, the pixel size in the header was lost. The real pixel size is 1.047 Å/pix. The dose rate is 0.9 e/Å^2/frame. Please use data/Movies/GainRef/190911_gainref.mrc as gain reference without any rotation or flipping.

Files:

Archives and downloads the selected files into an uncompressed zip file.

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Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. Nanodisc subtracted particles

Category:

picked particles - multiframe - processed

Image format:

MRCS

No. of images or tilt series:

403991

Image size:

(240, 240)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.1, 1.1)

Details:

Nanodisc-subtracted particles used for final reconstruction.

/data/Masks/for_refinement.mrc is the mask used for refinement.

/data/Masks/initial_subtraction.mrc is the mask used for initial subtraction of nanodisc density.

Files:

Archives and downloads the selected files into an uncompressed zip file.

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5. Nanodisc-reinstated particles

Category:

picked particles - multiframe - unprocessed

Image format:

MRCS

No. of images or tilt series:

403991

Image size:

(240, 240)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.1, 1.1)

Details:

Refined subtracted particles reverted to original particle images; suitable for calculating or refining the complex with nanodisc-signal contribution.

Files:

Archives and downloads the selected files into an uncompressed zip file.

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