EMPIAR-10335 - Apo-state Streptavidin [multiple data sets in MRCS format]

EMPIAR-10335

Apo-state Streptavidin [multiple data sets in MRCS format]

Publication:

High-yield monolayer graphene grids for near-atomic resolution cryoelectron microscopy

Han Y, Fan X, Wang H, Zhao F, Tully CG, Kong J, Yao N, Yan N

Proceedings of the National Academy of Sciences of the United States of America 117 (2019) 1009-1014

PMID: 31879346

DOI: 10.1073/pnas.1919114117

Related EMDB entry:

EMD-20907

Deposited:

2019-11-04

Released:

2019-12-20

Last modified:

2020-02-28

Imageset size:

922.40 GB

Imageset DOI:

10.6019/EMPIAR-10335

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
picked particles - single frame - processed

Image Sets

1. Unaligned 32-frame micrographs of 2.6 angstrom apo-state streptavidin on graphene

Category:

micrographs - multiframe

Image format:

MRCS

No. of images or tilt series:

1086

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.536, 0.536)

Details:

The micrographs are raw movies without any realignment, (accumulating 49 electron per squared Angstrom in a 2.4 second exposure).

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2. Polished particle stacks of 2.6 angstrom apo-state streptavidin on graphene

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

405

Image size:

(128, 128)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.072, 1.072)

Details:

Particle stacks are generated from Relion-3.0.6 Bayesian Polishing.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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