EMPIAR-10328 - Hedgehog receptor Patched (PTCH1) in complex with a conformation selective nanobody TI23 [stack of 307652 particles in MRCS format]

EMPIAR-10328

Hedgehog receptor Patched (PTCH1) in complex with a conformation selective nanobody TI23 [stack of 307652 particles in MRCS format]

Publication:

Hedgehog pathway activation through nanobody-mediated conformational blockade of the Patched sterol conduit

Zhang Y, Lu WJ, Bulkley DP, Liang J, Ralko A, Han S, Roberts KJ, Li A, Cho W, Cheng Y, Manglik A, Beachy PA

Proceedings of the National Academy of Sciences of the United States of America 117 (2020) 28838-28846

PMID: 33139559

DOI: 10.1073/pnas.2011560117

Related EMDB entry:

EMD-22689

Deposited:

2019-10-01

Released:

2021-11-08

Last modified:

2021-11-08

Imageset size:

75.22 GB

Imageset DOI:

10.6019/EMPIAR-10328

Experimental metadata:

Download xml json

Contains:

picked particles - multiframe - processed

Image Sets

1. Particle stack of Hedgehog receptor PTCH1 in complex with nanobody TI23 for final reconstruction

Category:

picked particles - multiframe - processed

Image format:

MRCS

No. of images or tilt series:

307652

Image size:

(256, 256)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.059, 1.059)

Details:

This is the particle stack for the final reconstruction of PTCH1-TI23 complex. The data sign is +1, white particle on dark background. The accompanying star file is exported from the last round of refinement in cryoSPARC2, with all the CTF parameters recorded.

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Quick Links

Talks and Tutorials

EMPIAR Quick tour

Statistics

Publications

Re-use case study

EMPIAR in the news

Claim entries to your ORCiD

COVID-19 Data Portal

EMDB

PDBe

BioImage Archive

EMNavigator@PDBj

PDBj

RCSB@PDBj

EMDataResoruce@RCSB,EMDB,NCMI

Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

See all citations

Protein Data Bank Japan News

[2024-11-20]

224 new PDB entries have been released on 2024-11-20.

[2024-11-01]

A new portal integrating genome variants and protein structures has released

[2024-10-30]

[wwPDB] Deprecation of FTP File Download Protocol on November 1, 2024

[2024-10-25]

PDBj luncheon seminar at APBJC 2024

[2024-10-17]

[wwPDB] The Nobel Prize in Chemistry 2024