EMPIAR-10328 - Hedgehog receptor Patched (PTCH1) in complex with a conformation selective nanobody TI23 [stack of 307652 particles in MRCS format]

EMPIAR-10328

Hedgehog receptor Patched (PTCH1) in complex with a conformation selective nanobody TI23 [stack of 307652 particles in MRCS format]

Publication:

Hedgehog pathway activation through nanobody-mediated conformational blockade of the Patched sterol conduit

Zhang Y, Lu WJ, Bulkley DP, Liang J, Ralko A, Han S, Roberts KJ, Li A, Cho W, Cheng Y, Manglik A, Beachy PA

Proceedings of the National Academy of Sciences of the United States of America 117 (2020) 28838-28846

PMID: 33139559

DOI: 10.1073/pnas.2011560117

Related EMDB entry:

EMD-22689

Deposited:

2019-10-01

Released:

2021-11-08

Last modified:

2021-11-08

Imageset size:

75.22 GB

Imageset DOI:

10.6019/EMPIAR-10328

Experimental metadata:

Download xml json

Contains:

picked particles - multiframe - processed

Image Sets

1. Particle stack of Hedgehog receptor PTCH1 in complex with nanobody TI23 for final reconstruction

Category:

picked particles - multiframe - processed

Image format:

MRCS

No. of images or tilt series:

307652

Image size:

(256, 256)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.059, 1.059)

Details:

This is the particle stack for the final reconstruction of PTCH1-TI23 complex. The data sign is +1, white particle on dark background. The accompanying star file is exported from the last round of refinement in cryoSPARC2, with all the CTF parameters recorded.

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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