EMPIAR-10318 - 200kV MicroED structure of FUS (37-42) SYSGYS solved from merged datasets at 0.65 A [8 diffraction images in SMV format]

EMPIAR-10318

200kV MicroED structure of FUS (37-42) SYSGYS solved from merged datasets at 0.65 A [8 diffraction images in SMV format]

Publication:

Programming Conventional Electron Microscopes for Solving Ultrahigh-Resolution Structures of Small and Macro-Molecules

Zhou H, Luo F, Luo Z, Li D, Liu C, Li X

Analytical Chemistry 91 (2019) 10996-11003

PMID: 31334636

DOI: 10.1021/acs.analchem.9b01162

Related EMDB entry:

EMD-0696

Deposited:

2019-09-19

Released:

2021-06-09

Last modified:

2021-06-09

Imageset size:

9.47 GB

Imageset DOI:

10.6019/EMPIAR-10318

Experimental metadata:

Download xml json

Contains:

diffraction images

Image Sets

1. 200kV MicroED data of FUS (37-42) SYSGYS

Category:

diffraction images

Image format:

SMV

No. of images or tilt series:

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(51.0, 51.0)

Details:

The data is collected on FEI Tecnai F20 200kV microscope. The electron wavelength is 0.02507 Angstrom. The final dataset is merged from 8 crystals. Each zip file contains one set of MicroED data (in SMV/img format) collected from one crystal. Each img file covers an 1 degree wedge (accumulating 0.0572 electron per squared Angstrom in a 5.72 seconds exposure). Since some data processing softwares (such as HKL2000) only support specific cameras used in X-ray crystallography, the pixel size of diffraction images has been set to 51 micrometers to imitate 4k x 4k ADSC Q210 camera, and the corresponding equivalent camera length has been saved in the header of img files and could be recognized by data processing softwares. The beam center information of each dataset is saved in the corresponding cec file and should be manually input to data processing softwares.

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