EMPIAR-10316 - Micrographs of E. coli 70S ribosomes collected at 100 keV using a hybrid pixel direct electron detector [127 multi-frame micrographs composed of 32 frames each in MRCS format]

EMPIAR-10316

Micrographs of E. coli 70S ribosomes collected at 100 keV using a hybrid pixel direct electron detector [127 multi-frame micrographs composed of 32 frames each in MRCS format]

Publication:

CryoEM at 100 keV: a demonstration and prospects

Naydenova K, McMullan G, Peet MJ, Lee Y, Edwards PC, Chen S, Leahy E, Scotcher S, Henderson R, Russo CJ

IUCrJ 6 (2019) 1086-1098

PMID: 31709064

DOI: 10.1107/S2052252519012612

Related EMDB entry:

EMD-10265

Deposited:

2019-08-27

Released:

2020-03-23

Last modified:

2019-08-28

Imageset size:

4.30 GB

Imageset DOI:

10.6019/EMPIAR-10316

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Raw multi-frame micrographs of E. coli 70S ribosomes recorded at 100 keV

Category:

micrographs - multiframe

Image format:

MRCS

No. of images or tilt series:

127

Image size:

(1030, 514)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(2.74, 2.74)

Details:

Unaligned movie frames (32) accumulating 16.6 electrons per squared Angstrom in a 2 second exposure.

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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