EMPIAR-10280 - Cryo-EM structure of calcium-bound TMEM16F in nanodisc with supplement of PIP2 [stack of 3823 particles in MRCS format]

EMPIAR-10280

Cryo-EM structure of calcium-bound TMEM16F in nanodisc with supplement of PIP2 [stack of 3823 particles in MRCS format]

Publication:

Cryo-EM Studies of TMEM16F Calcium-Activated Ion Channel Suggest Features Important for Lipid Scrambling

Feng S, Dang S, Han TW, Ye W, Jin P, Cheng T, Li J, Jan YN, Jan LY, Cheng Y

Cell reports 28 (2019) 567-579.e4

PMID: 31291589

DOI: 10.1016/j.celrep.2019.06.023

Related EMDB entry:

EMD-20246 EMD-20247

Deposited:

2019-05-27

Released:

2019-11-07

Last modified:

2019-11-07

Imageset size:

619.17 GB

Imageset DOI:

10.6019/EMPIAR-10280

Experimental metadata:

Download xml json

Contains:

picked particles - single frame - processed

Image Sets

1. Automated picked particle stack of calcium-bound TMEM16F in nanodisc with supplement of PIP2

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

3823

Image size:

(256, 256)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.059, 1.059)

Details:

All.star includes all automatically picked particles. Cl1.star and Cl2.star includes particles for final reconstruction of Cl1 and Cl2 respectively.

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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