EMPIAR-10277 - Tilt-schemes benchmarking for cryo electron tomography of HIV-1 CA-SP1 [multiple data sets in MRC format]

EMPIAR-10277

Tilt-schemes benchmarking for cryo electron tomography of HIV-1 CA-SP1 [multiple data sets in MRC format]

Publication:

Benchmarking tomographic acquisition schemes for high-resolution structural biology

Turoňová B, Hagen WJH, Obr M, Mosalaganti S, Beugelink JW, Zimmerli CE, Kräusslich HG, Beck M

Nature communications 11 (2020)

PMID: 32054835

DOI: 10.1038/s41467-020-14535-2

Related EMDB entry:

EMD-10207

Deposited:

2019-05-17

Released:

2020-03-02

Last modified:

2020-03-02

Imageset size:

45.57 GB

Imageset DOI:

10.6019/EMPIAR-10277

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Unsorted raw tilt-series for benchmarked tilt-schemes

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(3708, 3838)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.3269, 1.3269)

Details:

Raw, unsorted, non-dose-filtered tilt-series for 7 different tilt-schemes (5 tilt-series per each tilt-scheme). Each tilt-series contains 41 images - each image is an motion-corrected average of 10-20 frames.

Files:

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2. Tilt-series collected using the dose-symmetric scheme with 3 degrees tilt-step without any gold fiducials

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(None, 3838)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.3269, 1.3269)

Details:

Tilt-series acquired using the dose-symmetric tilt-scheme with 3 degrees tilt-step (tilt-range from -60 to +60 degrees) without any gold fiducials. The tilt-series are either not complete (i.e. have less than 41 images) and/or are cut in one dimension to remove any fiducials from the field of view. The trimming was done on the frame level to ensure that the final motion-corrected images do not profit from the presence of any fiducials. The tilt angles are contained in the header. The axis angle for all tilt-series was 85.5 degrees.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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