EMPIAR-10229 - Cryo-EM Reconstruction of apo EsCas13d [multiple data sets in MRCS format]

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EMPIAR-10229

Cryo-EM Reconstruction of apo EsCas13d [multiple data sets in MRCS format]

Publication:

Structural Basis for the RNA-Guided Ribonuclease Activity of CRISPR-Cas13d

Zhang C, Konermann S, Brideau N, Lotfy P, Wu X, Novick SJ, Strutzenberg T, Griffin P, Hsu PD, Lyumkis D

Cell 175 (2018) 212-223

PMID: 30241607

DOI: 10.1016/j.cell.2018.09.001

Related EMDB entry:

EMD-9015

Deposited:

2018-11-13

Released:

2018-11-23

Last modified:

2018-11-23

Imageset size:

66.89 GB

Imageset DOI:

10.6019/EMPIAR-10229

Experimental metadata:

Download xml json

Contains:

picked particles - single frame - unprocessed
picked particles - single frame - processed

Image Sets

1. stack of ~150k particles used for ab initio orientation assignment and reconstruction

Category:

picked particles - single frame - unprocessed

Image format:

MRCS

No. of images or tilt series:

154889

Image size:

(320, 320)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.73, 0.73)

Details:

This directory contains a stack and associated .star file of 154,889 particles. This stack was used as input to common-lines-based ab initio 3D orientation assignment and reconstruction in cryoSparc. The procedure resulted in the automated selection of 15,846 particles, from which a reconstruction was performed. This reconstruction is reported in the associated publication and in EMD-9015.

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2. stack of ~16k best particles from above

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

15846

Image size:

(256, 256)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.73, 0.73)

Details:

This directory contains a stack and associated .star file of the 15,846 best particles selected from the full stack. This stack represents the clean particles from above and reproduces the deposited map when subjected to an ab initio orientation assignment and reconstruction procedure.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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