EMPIAR-10164 - Cryo-electron tomography of immature HIV-1 dMACANC VLPs [43 multi-frame micrographs composed of 8 frames each in MRC format]

EMPIAR-10164

Cryo-electron tomography of immature HIV-1 dMACANC VLPs [43 multi-frame micrographs composed of 8 frames each in MRC format]

Publication:

An atomic model of HIV-1 capsid-SP1 reveals structures regulating assembly and maturation

Schur FK, Obr M, Hagen WJ, Wan W, Jakobi AJ, Kirkpatrick JM, Sachse C, Kräusslich HG, Briggs JA

Science (New York, N.Y.) 353 (2016) 506-508

PMID: 27417497

DOI: 10.1126/science.aaf9620

Related EMDB entry:

EMD-4015 EMD-3782

Deposited:

2018-02-19

Released:

2018-03-21

Last modified:

2022-07-14

Imageset size:

865.05 GB

Imageset DOI:

10.6019/EMPIAR-10164

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Compressed, unaligned, multi-frame micrographs of tilt series containing HIV-1 dMACANC virus like particles assembled in the presence of BVM.

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

Image size:

(7420, 7676)

Pixel type:

SIGNED BYTE

Pixel spacing:

(1.35, 1.35)

Details:

Information on all tilt series in corresponding .mrc.mdoc files (note that mdoc files refer to binned output, while raw frames are unbinned).
Dose per tilt series
TS_01 to TS_16: 3.0 e/A2/sec
TS_17 to TS_40: 3.5 e/A2/sec
TS_41 to TS_50: 3.1 e/A2/sec
TS_51 to TS_63: 3.0 e/A2/sec

The frames are compressed, without any alignment, and gain corrected.

The subset of data (5 tomograms) that was used for testing in Turonova et al, J Struct Biol. 2017 Sep;199(3):187-195. doi: 10.1016/j.jsb.2017.07.007 is
TS_01
TS_03
TS_43
TS_45
TS_54

Part of the dataset is 8 frames per image, part of the dataset is 10 frames per image. All frames were used.

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