EMPIAR-10105 - FIB-SEM of a dividing cell at 5.7 min after anaphase onset [2620 multi-frame micrographs composed of 1 frames each in TIFF format]

EMPIAR-10105

FIB-SEM of a dividing cell at 5.7 min after anaphase onset [2620 multi-frame micrographs composed of 1 frames each in TIFF format]

Publication:

Postmitotic nuclear pore assembly proceeds by radial dilation of small membrane openings

Otsuka S, Steyer AM, Schorb M, Hériché JK, Hossain MJ, Sethi S, Kueblbeck M, Schwab Y, Beck M, Ellenberg J

Nature structural & molecular biology 25 (2017) 21-28

PMID: 29323269

DOI: 10.1038/s41594-017-0001-9

Related EMDB entry:

Deposited:

2017-05-25

Released:

2017-11-28

Last modified:

2021-10-25

Imageset size:

63.02 GB

Imageset DOI:

10.6019/EMPIAR-10105

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. FIB-SEM images of a dividing cell at 5.7 min after anaphase onset

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2620

Image size:

(4190, 6164)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(5.0, 8.0)

Details:

The pixel size in xy was set to be 5 nm and the FIB-milling was done every 8 nm. The acquired raw FIB-SEM images were aligned using TrackEM2 (Cardona et al., PloS one, e38011, 2012) in ImageJ (http://rsbweb.nih.gov/ij/). After alignment, the stack was cropped, gray levels were inverted and the images were smoothened with a mean filter (kernel size: 15 × 15 nm). We used the first half of the cell for segmentation.

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