EMPIAR-10057 - Volta phase plate in-focus dataset of T20S proteasome [158 multi-frame micrographs composed of 12 frames each in MRC format]

EMPIAR-10057

Volta phase plate in-focus dataset of T20S proteasome [158 multi-frame micrographs composed of 12 frames each in MRC format]

Publication:

Cryo-EM single particle analysis with the Volta phase plate

Danev R, Baumeister W

eLife 5 (2016)

PMID: 26949259

DOI: 10.7554/eLife.13046

Related EMDB entry:

EMD-3347

Deposited:

2016-02-29

Released:

2016-03-16

Last modified:

2016-03-16

Imageset size:

53.99 GB

Imageset DOI:

10.6019/EMPIAR-10057

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Volta phase plate in-focus unaligned multi-frame micrographs of T20S proteasome

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

158

Image size:

(3838, 3710)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.35, 1.35)

Details:

Particle coordinates are included as BOX files. Data acquisition settings are listed in the "acquisition_parameters.txt" file.

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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