EMPIAR-10013 - Structure of β-galactosidase at 3.2-Å resolution obtained by cryo-electron microscopy [multiple data sets in MRC and DM4 formats]

EMPIAR-10013

Structure of β-galactosidase at 3.2-Å resolution obtained by cryo-electron microscopy [multiple data sets in MRC and DM4 formats]

Publication:

Structure of beta-galactosidase at 3.2-A resolution obtained by cryo-electron microscopy

Bartesaghi A, Matthies D, Banerjee S, Merk A, Subramaniam S

Pnas 111 (2014) 11709-11714

PMID: 25071206

DOI: doi:10.1073/pnas.1402809111

Related EMDB entry:

EMD-5995

Deposited:

2014-07-23

Released:

2014-08-07

Last modified:

2014-08-07

Imageset size:

442.52 GB

Imageset DOI:

10.6019/EMPIAR-10013

Experimental metadata:

Download xml json

Version history:

2015-07-01

REL

Directory structure reorganized. Entry has now full set of micrographs including frame alignment shifts, particle locations and defoci.

Contains:

micrographs - multiframe

Image Sets

1. Full set of multi-frame micrographs

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

509

Image size:

(7420, 7676)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.6375, 0.6375)

Details:

Full set of multiframe micrographs related to the dataset:10012. Related PDB: 3J7H.
Please note that the number of frames varies between [5, 10, 25 and 38]!
Although the image files are in MRC format, they have been compressed to save space. They can be uncompresssed using for example: for f in *.tbz; do tar -xjf $f -C mrc/ ; done
Image files are numbered: BGal_000000_frames.tbz to BGal_000508_frames.tbz
Frame alignment shifts in IMOD's transform format: BGal_000000.xf to BGal_000508.xf
Picked particle locations in EMAN's boxer format: BGal_000000.box to BGal_000508.box
Defocus per image in Angstroms: BGal_defocus.txt
Additional details:
Cs : 2.7
Anisotropic magnification (if known) ? : N/A
frame rate : 2.5 to .33 frames/s
dose per frame : 1.2 to 9 e-/A^2
alignment method/software : 10.1073/pnas.1402809111
which frame used for alignment reference: middle frame
particle selection software : 10.1073/pnas.1402809111

Files:

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2. Sample set of multi-frame micrographs

Category:

micrographs - multiframe

Image format:

DM4

No. of images or tilt series:

Image size:

(7420, 7676)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.6375, 0.6375)

Details:

Selection of 5 multiframe micrographs related to the dataset:10012. Related PDB: 3J7H.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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