EMPIAR-10863 - Cryo-EM of recombinant Goslar chimallin [2889 multi-frame micrographs composed of 44 frames each in TIFF format]

EMPIAR-10863

Cryo-EM of recombinant Goslar chimallin [2889 multi-frame micrographs composed of 44 frames each in TIFF format]

Publication:

Architecture and self-assembly of the jumbo bacteriophage nuclear shell

Laughlin TG, Deep A, Prichard AM, Seitz C, Gu Y, Enustun E, Suslov S, Khanna K, Birkholz EA, Armbruster E, McCammon JA, Amaro RE, Pogliano J, Corbett KD, Villa E

bioRxiv (2022)

DOI: 10.1101/2022.02.14.480162

Related EMDB entry:

EMD-25396 EMD-25395 EMD-25394

Deposited:

2021-11-07

Released:

2022-07-26

Last modified:

2022-07-26

Imageset size:

432.87 GB

Imageset DOI:

10.6019/EMPIAR-10863

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned frames as unormalized LZW-TIFF

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2889

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.8452, 0.8452)

Details:

Gain reference supplied in data/frames as MRC and requires a 270 degree CW rotation prior to application to TIFs. Each frame-series is 44 frames with cumulative fluence of 40 electrons per square Angstrom. Particle metadata for the localized tetramer stage in RELION-v3.1 format and for the fully expanded protomer stage in cryoSPARC-v3.1 format in data/particle_metadata.

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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