EMPIAR-10679 - Subtomograms of nucleosomes extracted from cryo-tomograms of Drosophila melanogaster embryos [1 subtomograms in EM format]

EMPIAR-10679

Subtomograms of nucleosomes extracted from cryo-tomograms of Drosophila melanogaster embryos [1 subtomograms in EM format]

Publication:

HEMNMA-3D: Cryo Electron Tomography Method Based on Normal Mode Analysis to Study Continuous Conformational Variability of Macromolecular Complexes

Harastani M, Eltsov M, Leforestier A, Jonic S

Frontiers in molecular biosciences 8 (2021)

PMID: 34095222

DOI: 10.3389/fmolb.2021.663121

Related EMDB entry:

EMD-12699

Deposited:

2021-03-31

Released:

2021-06-18

Last modified:

2021-06-18

Imageset size:

698.70 MB

Imageset DOI:

10.6019/EMPIAR-10679

Experimental metadata:

Download xml json

Contains:

subtomograms

Image Sets

1. Unaligned subtomograms of nucleosomes extracted from in situ cryo-tomograms of Drosophila melanogaster embryonic brain

Category:

subtomograms

Image format:

No. of images or tilt series:

Image size:

(None, None)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(4.4, 4.4)

Details:

Tilt series were recorded using the dose-symmetric recording scheme of Serial EM software at a nominal magnification of 64000 and a target defocus of -3.5 ˚ µm. The total dose was set to 91.5 e−/ A˚
A marker-less tilt series alignment was done in IMOD. Nucleosomes were manually picked in IMOD, and 64x64x64 voxel subtomograms (voxel size of 4.4 A) were extracted from the volumes.

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Citations

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PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

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