EMPIAR-10664 - Disease variant M854K MDA5-dsRNA filaments in complex with ADP-AlF4 [2177 multi-frame micrographs composed of 60 frames each in TIFF format]

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EMPIAR-10664

Disease variant M854K MDA5-dsRNA filaments in complex with ADP-AlF4 [2177 multi-frame micrographs composed of 60 frames each in TIFF format]

Publication:

MDA5 disease variant M854K prevents ATP-dependent structural discrimination of viral and cellular RNA

Yu Q, Herrero Del Valle A, Singh R, Modis Y

Nature communications 12 (2021)

PMID: 34795277

DOI: 10.1038/s41467-021-27062-5

Related EMDB entry:

EMD-12288 EMD-12294

Deposited:

2021-03-07

Released:

2021-11-12

Last modified:

2022-02-25

Imageset size:

2.76 TB

Imageset DOI:

10.6019/EMPIAR-10664

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of MDA5 variant M854K bound to dsRNA and ADP-ALF4

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2177

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.084, 1.084)

Details:

Particle positions used for final reconstruction are in corresponding *particles.star files.
Each raw movie contain 60 unaligned frames (accumulating 29.6 electrons per square Å in a 60s exposure).

Files:

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Citations

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PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

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Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

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Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

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