EMPIAR-10443 - Mechanism of Ribosome Rescue by Alternative Release Factor B [multiple data sets in MRC format]

EMPIAR-10443

Mechanism of Ribosome Rescue by Alternative Release Factor B [multiple data sets in MRC format]

Publication:

Mechanism of Ribosome Rescue by Alternative Release Factor B

Chan K-H, Petrychenko V, Mueller C, Maracci C, Holtkamp W, Wilson DN, Fischer N, Rodnina MV

Nature Communications 11 (2020)

PMID: 27906160

DOI: 10.1038/s41467-020-17853-7

Related EMDB entry:

EMD-10905 EMD-10908 EMD-10906 EMD-10907

Deposited:

2020-06-11

Released:

2020-08-18

Last modified:

2020-08-18

Imageset size:

846.32 GB

Imageset DOI:

10.6019/EMPIAR-10443

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Motion-corrected, dose-weighted micrographs & polished particles of P+9 E. coli ribosome-ArfB complex

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

4750

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.16, 1.16)

Details:

Note: Micrographs were obtained from movie-stacks of 40 frames à 1.25 e/A^2 by global motion-correction and dose-weighting using MotionCor2 in Relion, "shiny" particles were obtained by Baysian polishing in Relion 3.0
- Micrographs .star-file: "micrographs_ctf.star"
- Particles .star-file for all particles: "particles_all.star"
- Particles .star-file for particles of the P+9 stalled ribosome complex (EMD-10905): "particles_P+9_stalled_complex.star"
- Particles .star-file for particles of P+9 ArfB-ribosome complex in the post-hydrolysis state (EMD-10906): "particles_P+9 post-hydrolysis state.star"
- Particles .star-file for particles of P+9 ArfB-ribosome complex with P/E hybrid tRNA in the post-hydrolysis state (EMD-10907): "particles_P+9 post-hydrolysis state with PE hybrid tRNA.star"

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2. Motion-corrected, dose-weighted micrographs & extracted particles of P+0 E. coli ribosome-ArfB complex

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

3633

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.072, 1.072)

Details:

Note: Micrographs were obtained from movie-stacks of 10 frames à 2.5 e/A^2 by global motion-correction and dose-weighting using MotionCor2 in Relion
- Micrographs .star-file: "micrographs_ctf.star"
- Particles .star-file for particles of P+0 ArfB-ribosome complex in the post-hydrolysis state (EMD-10908): "particles_P+0 post-hydrolysis state.star"

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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