EMPIAR-12890 - Structure of in-vivo formed alpha-synuclein fibrils purified from a M83+/- mouse brain injected with recombinant 1B fibrils [multiple data sets in EER, MRC and MRCS formats]

EMPIAR-12890

Structure of in-vivo formed alpha-synuclein fibrils purified from a M83+/- mouse brain injected with recombinant 1B fibrils [multiple data sets in EER, MRC and MRCS formats]

Publication:

1.94 Å structure of synthetic α-synuclein fibrils seeding MSA neuropathology

Burger D, Kashyrina M, Lewis AJ, De Nuccio F, Mohammed I, de La Seiglière H, van den Heuvel L, Verchère J, Feuillie C, Berbon M, Arotçarena M, Retailleau A, Bezard E, Laferrière F, Loquet A, Bousset L, Baron T, Lofrumento DD, De Giorgi F, Stahlberg H, Ichas F

bioRxiv (2024)

DOI: 10.1101/2024.07.01.601498

Related EMDB entry:

EMD-54402

Deposited:

2025-07-15

Released:

2025-08-04

Last modified:

2025-08-04

Imageset size:

18.14 GB

Imageset DOI:

10.6019/EMPIAR-12890

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame
picked particles - single frame - unprocessed

Image Sets

1. Unaligned multi-frame micrographs (EER), pre-selected manually for the presence of twisting fibrils.

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

Image size:

(16384, 16384)

Pixel type:

SIGNED BYTE

Pixel spacing:

(None, None)

Details:

---

Files:

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2. Motion-corrected micrographs (MRC), pre-selected manually for the presence of twisting fibrils.

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

Image size:

(4096, 4096)

Pixel type:

16 BIT FLOAT

Pixel spacing:

(0.732, 0.732)

Details:

Micrographs which were used for particle picking in RELION, motion-corrected using RELION's own implementation of MOTIONCOR. Five files are listed for each micrograph: (1) the aligned MRC, (2) the micrograph power spectrum used for CTF correction ("_PS.mrc"), (3) the shifts used in motion correction ("_shifts.eps"), (4) the shifts and header in .star format (".star") and (5) the log file of the motion correction.

Files:

Archives and downloads the selected files into an uncompressed zip file.

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Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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3. Extracted particles of manually picked fibrils (box size 800, binning 2, inter-box distance of 14.4A).

Category:

picked particles - single frame - unprocessed

Image format:

MRCS

No. of images or tilt series:

Image size:

(400, 400)

Pixel type:

16 BIT FLOAT

Pixel spacing:

(1.464, 1.464)

Details:

Extracted particles of manually picked fibrils (box size 800, binning 2, inter-box distance of 14.4A), before purification by a series of 2D classification and selection. Two files are listed for each micrograph: (1) the mrcs file with dimensions (N,400,400) where N is the number of particles extracted from that micrograph, and (2) the star file listing the coordinates of each particle ("_extract.star").

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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