EMPIAR-12458
Dataset 2: Complex 2,3 and 4: 30S ribosome with IF1/2/3, tRNA, and GE81112 in complex preparation reaction [6172 multi-frame micrographs composed of 19 frames each in MRC format]
Publication:

A binding site for the antibiotic GE81112 in the ribosomal mRNA channel

Schedlbauer A, Han X, van Bakel W, Kaminishi T, Ochoa-Lizarralde B, Iturrioz I, Çapuni R, Parry R, Zegarra R, Gil-Carton D, López-Alonso JP, Sanz KB, Brandi L, Gualerzi CO, Fucini P, Connell SR

bioRxiv (2024)

Related EMDB entry:
Deposited:
2024-10-31
Released:
2025-08-01
Last modified:
2025-08-01
Imageset size:
2.32 TB
Imageset DOI:
Experimental metadata:
Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (FoilHole_*_Fractions.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

Original size:   3,842,644,000 kilobytes
Compressed size: 2,491,891,390 kilobytes
Contains:
  • micrographs - multiframe
1. unaligned multiframe movies from dataset 2: 30S + IF1/2/3 +mRNA + tRNA GE81112
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
6172
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(1.113, 1.113)
Details:
unaligned multiframe movies from dataset 2: 30S + IF1/2/3 +mRNA + tRNA GE81112. Total Exposure (e-/ Å 2)
44.08; Voltage (kV) 300
Files:
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Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)
Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)
Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)
Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)