EMPIAR-12410 - Cryo-EM structures of SV2A in complex with antiepileptic drugs and botulinum neurotoxin A2 receptor-binding domain [multiple data sets in TIFF format]

EMPIAR-12410

Cryo-EM structures of SV2A in complex with antiepileptic drugs and botulinum neurotoxin A2 receptor-binding domain [multiple data sets in TIFF format]

Publication:

Structural basis for antiepileptic drugs and botulinum neurotoxin recognition of SV2A

Yamagata A, Ito K, Suzuki T, Dohmae N, Terada T, Shirouzu M

Nature communications 15 (2024)

PMID: 38637505

DOI: 10.1038/s41467-024-47322-4

Related EMDB entry:

EMD-36934 EMD-36933 EMD-36395 EMD-36397 EMD-36398 EMD-36394 EMD-36617 EMD-36935 EMD-36396 EMD-36616

Deposited:

2024-09-05

Released:

2025-06-26

Last modified:

2025-06-26

Imageset size:

7.28 TB

Imageset DOI:

10.6019/EMPIAR-12410

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. SC2A-HcA2-LEV

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6518

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

- 8JLC / EMD-36392
- 8JLE / EMD-36394
- 8JS8 / EMD-36616
- 8JLH / EMD-36397

- Microscope: FEI TITAN Krios
- Detector: Gatan K3 BIOQUANTUM
- Acceleration voltage (kV): 300
- The pixel size in the image header (1 Å/px) is wrong. 0.83 Å/px is the calibrated value.
- Spherical aberration (Cs) : 2.7
- Nominal Defocus (μm): 0.8 - 2.0
- Average electron dose per image (e-/A2): 50.8
- Please use 'gain.mrc' as a gain reference without rotation and y-flipping.

Files:

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2. SV2-LEV

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

10001

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

- 8JLI / EMD-36398

- Microscope: FEI TITAN Krios
- Detector: Gatan K3 BIOQUANTUM
- Acceleration voltage (kV): 300
- The pixel size in the image header (1 Å/px) is wrong. 0.83 Å/px is the calibrated value.
- Spherical aberration (Cs) : 2.7
- Nominal Defocus (μm): 0.8 - 2.0
- Average electron dose per image (e-/A2): 50.2
- Please use 'gain.mrc' as a gain reference without rotation and y-flipping.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. SV2A-HcA2

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5930

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

- 8JLF / EMD-36395
- 8JLG / EMD-36396
- 8JS9 / EMD-36617

- Microscope: FEI TITAN Krios
- Detector: Gatan K3 BIOQUANTUM
- Acceleration voltage (kV): 300
- The pixel size in the image header (1 Å/px) is wrong. 0.83 Å/px is the calibrated value.
- Spherical aberration (Cs) : 2.7
- Nominal Defocus (μm): 0.8 - 2.0
- Average electron dose per image (e-/A2): 50.0
- Please use 'gain.mrc' as a gain reference without rotation and y-flipping.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. SV2A-HcA2-BRV

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6001

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

- 8K77 / EMD-36933, EMD-36934, EMD-36935

- Microscope: FEI TITAN Krios
- Detector: Gatan K3 BIOQUANTUM
- Acceleration voltage (kV): 300
- The pixel size in the image header (1 Å/px) is wrong. 0.83 Å/px is the calibrated value.
- Spherical aberration (Cs) : 2.7
- Nominal Defocus (μm): 0.8 - 2.0
- Average electron dose per image (e-/A2): 50.0
- Please use 'gain.mrc' as a gain reference without rotation and y-flipping.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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