EMPIAR-12338 - CryoEM of E. coli RNA polymerase and lambda PR promoter DNA at equilibrium at 5 degrees Celsius [2764 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-12338

CryoEM of E. coli RNA polymerase and lambda PR promoter DNA at equilibrium at 5 degrees Celsius [2764 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

Early intermediates in bacterial RNA polymerase promoter melting visualized by time-resolved cryo-electron microscopy

Saecker RM, Mueller AU, Malone B, Chen J, Budell WC, Dandey VP, Maruthi K, Mendez JH, Molina N, Eng ET, Yen LY, Potter CS, Carragher B, Darst SA

Nature structural & molecular biology (2024)

PMID: 38951624

DOI: 10.1038/s41594-024-01349-9

Related EMDB entry:

EMD-41439 EMD-41448 EMD-41437 EMD-41433

Deposited:

2024-10-04

Released:

2024-12-05

Last modified:

2024-12-05

Imageset size:

1.16 TB

Imageset DOI:

10.6019/EMPIAR-12338

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned raw movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2764

Image size:

(7676, 7420)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.096, 1.096)

Details:

FEI Titan Krios + Gatan K2, super-resolution, energy filter 20 eV slit, Cs 0.001 mm; accelerating voltage 300 kV; dose rate 6.76 e-/ A^2/s; frame time 0.2 s (50 frames) total exposure of 10 s, total dose of 67.63, e-/A^2, pixel size 1.096 A/px(detector); pixel size 0.548 A/px(image); defocus range -1.0 to -2.5 um; gain reference is 18aug21g_22103126_05_7420x7676_norm_0_mod.mrc (references folder).

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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