EMPIAR-12338 - CryoEM of E. coli RNA polymerase and lambda PR promoter DNA at equilibrium at 5 degrees Celsius [2764 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-12338

CryoEM of E. coli RNA polymerase and lambda PR promoter DNA at equilibrium at 5 degrees Celsius [2764 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

Early intermediates in bacterial RNA polymerase promoter melting visualized by time-resolved cryo-electron microscopy

Saecker RM, Mueller AU, Malone B, Chen J, Budell WC, Dandey VP, Maruthi K, Mendez JH, Molina N, Eng ET, Yen LY, Potter CS, Carragher B, Darst SA

Nature structural & molecular biology (2024)

PMID: 38951624

DOI: 10.1038/s41594-024-01349-9

Related EMDB entry:

EMD-41439 EMD-41448 EMD-41437 EMD-41433

Deposited:

2024-10-04

Released:

2024-12-05

Last modified:

2024-12-05

Imageset size:

1.27 TB

Imageset DOI:

10.6019/EMPIAR-12338

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned raw movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2764

Image size:

(7676, 7420)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.096, 1.096)

Details:

FEI Titan Krios + Gatan K2, super-resolution, energy filter 20 eV slit, Cs 0.001 mm; accelerating voltage 300 kV; dose rate 6.76 e-/ A^2/s; frame time 0.2 s (50 frames) total exposure of 10 s, total dose of 67.63, e-/A^2, pixel size 1.096 A/px(detector); pixel size 0.548 A/px(image); defocus range -1.0 to -2.5 um; gain reference is 18aug21g_22103126_05_7420x7676_norm_0_mod.mrc (references folder).

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

See all citations

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