EMPIAR-11662 - Single particle cryo-EM dataset of Yarrowia lipolytica mitochondrial complex I [2540 multi-frame micrographs composed of 25 frames each in MRC format]

EMPIAR-11662

Single particle cryo-EM dataset of Yarrowia lipolytica mitochondrial complex I [2540 multi-frame micrographs composed of 25 frames each in MRC format]

Publication:

Mitochondrial complex I structure reveals ordered water molecules for catalysis and proton translocation

Grba DN, Hirst J

Nature structural & molecular biology 27 (2020) 892-900

PMID: 32747785

DOI: 10.1038/s41594-020-0473-x

Related EMDB entry:

EMD-10815 EMD-10816

Deposited:

2023-08-02

Released:

2024-06-27

Last modified:

2024-06-27

Imageset size:

377.87 GB

Imageset DOI:

10.6019/EMPIAR-11662

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies (FoilHole*.mrc) in this entry were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

Compression was carried out by separating (“un-normalizing”) the gain.
The details of the method are described in
https://relion.readthedocs.io/en/release-3.1/Reference/MovieCompression.html#gain-normalised-movies.

Please apply the gain reference (gain-reference.mrc) during processing.

Original size:   3,531,937,314 kilobytes
Compressed size:   396,224,892 kilobytes

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of wild-type Yarrowia lipolytica mitochondrial complex I from an as-prepared sample solubilised in DDM.

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

2540

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.055, 1.055)

Details:

Unaligned multi-frame micrographs of wild-type Yarrowia lipolytica mitochondrial complex I from an as-prepared sample solubilised in DDM. Frozen gold grids treated with PEGylated thiol reagent. Images (MRC) were collected with a 300 keV FEI Titan Krios on a Gatan K2 Summit detector, using counting mode and a Gatan BioContinuum energy filter (20 eV). The C2 and objective lens apertures were set to 50 and 100 µm, respectively. 10 s exposures were captured in 25 frames with a total dose of ~44 e/Å-2. A pixel size of 1.055 Å/pixel was used.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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