EMPIAR-11525 - Ex vivo murine AApoAII amyloid fibril [3083 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-11525

Ex vivo murine AApoAII amyloid fibril [3083 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Insights into the Structural Basis of Amyloid Resistance Provided by Cryo-EM Structures of AApoAII Amyloid Fibrils

Andreotti G, Baur J, Ugrina M, Pfeiffer PB, Hartmann M, Wiese S, Miyahara H, Higuchi K, Schwierz N, Schmidt M, Fändrich M

Journal of molecular biology 436 (2024)

PMID: 38199491

DOI: 10.1016/j.jmb.2024.168441

Related EMDB entry:

EMD-17104 EMD-17105

Deposited:

2023-03-31

Released:

2024-03-18

Last modified:

2024-03-18

Imageset size:

510.55 GB

Imageset DOI:

10.6019/EMPIAR-11525

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of ex vivo murine AApoAII fibrils

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3083

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(None, None)

Details:

Krios1 Quantum-K2
Cs 2.7
300kV EFTEM nanpprobe
x130k spot 8 beam size 620nm
c2=50 objective_aperture=100

GIF-K2 counting
12s exposure 40 frames
total dose at the sample 42.70e/A2
1 pixel = 1.04A
dose rate ~3 e/pix/s

defocus range -1.2 to -2.5

Gain reference is already applied to the micrographs and the NOTE.txt contains the EM information.
Thumbnails and power spectra (/data/thumbs) are provided, as well as the defect files (/data/data).
The ManualPick folder contains the coordinates for two different fibril morphologies. Coordinates files are divided in part_1 and part_2 for each morphologies and they are connected to micrographs with different defect files.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

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Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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