EMPIAR-11525 - Ex vivo murine AApoAII amyloid fibril [3083 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-11525

Ex vivo murine AApoAII amyloid fibril [3083 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Insights into the Structural Basis of Amyloid Resistance Provided by Cryo-EM Structures of AApoAII Amyloid Fibrils

Andreotti G, Baur J, Ugrina M, Pfeiffer PB, Hartmann M, Wiese S, Miyahara H, Higuchi K, Schwierz N, Schmidt M, Fändrich M

Journal of molecular biology 436 (2024)

PMID: 38199491

DOI: 10.1016/j.jmb.2024.168441

Related EMDB entry:

EMD-17104 EMD-17105

Deposited:

2023-03-31

Released:

2024-03-18

Last modified:

2024-03-18

Imageset size:

548.20 GB

Imageset DOI:

10.6019/EMPIAR-11525

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of ex vivo murine AApoAII fibrils

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3083

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(None, None)

Details:

Krios1 Quantum-K2
Cs 2.7
300kV EFTEM nanpprobe
x130k spot 8 beam size 620nm
c2=50 objective_aperture=100

GIF-K2 counting
12s exposure 40 frames
total dose at the sample 42.70e/A2
1 pixel = 1.04A
dose rate ~3 e/pix/s

defocus range -1.2 to -2.5

Gain reference is already applied to the micrographs and the NOTE.txt contains the EM information.
Thumbnails and power spectra (/data/thumbs) are provided, as well as the defect files (/data/data).
The ManualPick folder contains the coordinates for two different fibril morphologies. Coordinates files are divided in part_1 and part_2 for each morphologies and they are connected to micrographs with different defect files.

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