EMPIAR-10875 - Cryo-EM structure of pioneer factor Cbf1 bound to the nucleosome [6339 multi-frame micrographs composed of 51 frames each in TIFF format]

EMPIAR-10875

Cryo-EM structure of pioneer factor Cbf1 bound to the nucleosome [6339 multi-frame micrographs composed of 51 frames each in TIFF format]

Publication:

Basic helix-loop-helix pioneer factors interact with the histone octamer to invade nucleosomes and generate nucleosome-depleted regions

Donovan BT, Chen H, Eek P, Meng Z, Jipa C, Tan S, Bai L, Poirier MG

Molecular cell 83 (2023) 1251-1263.e6

PMID: 36996811

DOI: 10.1016/j.molcel.2023.03.006

Related EMDB entry:

EMD-25406

Deposited:

2021-11-10

Released:

2023-05-23

Last modified:

2023-05-23

Imageset size:

4.09 TB

Imageset DOI:

10.6019/EMPIAR-10875

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of Cbf1/nucleosome complex

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6339

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.644, 0.644)

Details:

Voltage 300 keV, Cs 2.7 mm, total dose 50 e-/Å^2, super-resolution pixel size 0.644 Å/px. The TIFF movies are not gain-corrected, the gain-reference image for the dataset is SuperRef_stack2_00000_X-1Y-1-0 provided in DM4 and MRC formats.

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Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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