Electron Microscopy Public Image Archive
Electron Microscopy Public Image Archive
Due to a storage failure, some datasets are currently inaccessible or incomplete (marked as Incomplete dataset).
We are pleased to report that the following entries have been restored:
EMPIAR-10577 (New)We are still accepting requests for datasets you would like us to download first (email, inquiry).
We apologize for the inconvenience and appreciate your understanding.
EMPIAR-PDBj services will be suspended during the following period for a legally required inspection of electrical equipment.
During this period, the website and data downloads from EMPIAR-PDBj will be unavailable.
Please use EMPIAR-EBI instead.
We apologize for any inconvenience.
The EMPIAR-PDBj team at Osaka University assists Asian EM researchers with the transfer of big EM image data to EMPIAR. Instead of sending the data directly to the EBI (UK) via the internet, hard drives can also be sent to Osaka University by postal mail or via a courier service. As an alternative, internet transfer to our server in Osaka is also available. If you would like to take advantage of our submission services, please contact us first by e-mail before sending the data to us.
| Release date | Imageset | Title | Authors and references | Size | Resolution |
|---|---|---|---|---|---|
| 2025-05-10 |  |
Unaligned multi-frame micrographs of DNA polymerase theta with 3′-overhang DNA with 9-nt polyT and 6-nt MH sequence [10005 multi-frame micrographs composed of 50 frames each in TIFF format] | Ito F, Li Z, Khant HA, Chen XS [Pubmed: 40253368] [DOI: 10.1038/s41467-025-58441-x] |
2.7 TB | 3.1 Å |
| 2024-07-11 |  |
Unaligned multi-frame micrographs of SARS-CoV-2 Nsp15 in apo form and in complex with poly(U)-containing dsRNA [22419 multi-frame micrographs composed of 2051 frames each in EER format] | Ito F, Yang H, Zhou ZH, Chen XS [Pubmed: 38613382] [DOI: 10.1093/procel/pwae009] |
24.5 TB | 2.33 - 3.25 Å |
| 2025-01-09 |  |
Unaligned multi-frame micrographs of the mu opioid receptor bound to a nanobody antagonist NbE [5939 multi-frame micrographs composed of 44 frames each in MRC format] | Yu J, Kumar A, Zhang X, Martin C, Holsbeeck KV, Raia P, Koehl A, Laeremans T, Steyaert J, Manglik A, Ballet S, Boland A, Stoeber M [Pubmed: 38106026] [DOI: 10.1101/2023.12.06.570395] |
8.0 TB | 3.2 Å |
| 2026-04-27 |  |
Unbend:Local correction of beam-induced sample motion in cryo-EM images using 3D spline model [1 multi-frame micrographs composed of 30 frames each in TIFF format] | Lingli Kong XZ, Johannes Elferich JE, Ximena Zottig XZ, Nikolaus Grigorieff NG | 18.1 GB | — |
| 2026-04-27 |  |
Unbend:Local correction of beam-induced sample motion in cryo-EM images using 3D spline model [1 multi-frame micrographs composed of 30 frames each in TIFF format] | Lingli Kong XZ, Johannes Elferich JE, Ximena Zottig XZ, Nikolaus Grigorieff NG | 11.0 GB | — |
| 2026-05-01 |  |
Unbend:Local correction of beam-induced sample motion in cryo-EM images using 3D spline model [1 multi-frame micrographs composed of 75 frames each in TIFF format] | Lingli Kong XZ, Johannes Elferich JE, Ximena Zottig XZ, Nikolaus Grigorieff NG | 42.7 GB | — |
| 2025-07-25 |  |
Uncharacterized Q8U0N8 protein from Pyrococcus furiosus [6465 multi-frame micrographs composed of 50 frames each in EER format] | Schweke H, Pacesa M, Levin T, Goverde CA, Kumar P, Duhoo Y, Dornfeld LJ, Dubreuil B, Georgeon S, Ovchinnikov S, Woolfson DN, Correia BE, Dey S, Levy ED [Pubmed: 38325366] [DOI: 10.1016/j.cell.2024.01.022] |
4.5 TB | 2.79 Å |
| 2024-03-18 |  |
Unseeded Aβ(1-40) amyloid fibrils [4628 multi-frame micrographs composed of 918 frames each in EER format] | Pfeiffer PB, Schmidt M, Fändrich M [Pubmed: 38158175] [DOI: 10.1016/j.jmb.2023.168422] |
1.9 TB | 2.59 - 2.73 Å |
| 2016-07-06 |  |
Unsupervised single-particle deep classification via statistical manifold learning [multiple data sets in MRC format] | Wu J, Ma YB, Congdon C, Brett B, Chen S, Xu Y, Ouyang Q, Mao Y | 28.2 GB | — |
| 2017-05-15 |  |
Untilted Single-Particle CryoEM of Highly Preferred Orientated Influenza Hemagglutinin Trimer [multiple data sets in MRC format] | Tan YZ, Lyumkis D [Pubmed: 28671674] [DOI: 10.1038/nmeth.4347] |
247.5 GB | 4.2 Å |
| 2024-03-19 |  |
Unveiling the ultrastructural landscape of extracellular matrix via lift-out cryo-FIBSEM and cryo-ET [multiple data sets in TIFF and MRC formats] | Zens B., Fäßler F., Hansen J.M., Hauschild R., Datler J., Hodirnau V.V., Zheden V., Alanko J., Sixt M., Schur F.K.M. [Pubmed: 38506714] [DOI: 10.1083/jcb.202309125] |
183.0 GB | — |
| 2021-11-08 |  |
VHUT-cryo-FIB, a method to fabricate frozen-hydrated lamella of tissue specimen for in situ cryo-electron tomography [13 multi-frame micrographs composed of 30 frames each in TIFF format] | Zhang J [Pubmed: 34174447] [DOI: 10.1016/j.jsb.2021.107763] |
112.1 GB | 18.0 Å |
| 2016-08-15 |  |
VPP subtomogram averaging [11 class averages in MRC format] | Khoshouei M, Pfeffer S, Baumeister W, Foerster F, Danev R [Pubmed: 27235783] [DOI: 10.1016/j.jsb.2016.05.009] |
33.9 GB | 9.6 Å |
| 2023-05-22 |  |
Venous puncture wound thrombi, 1, 5, 20 min post-puncture, 100 nm XY raw images, 20 nm XY pixels every 20 micons, 3 nm wide area TEM montages at selected depth [15 multi-frame micrographs composed of 1 frames each in DM4 format] | Storrie B, Leapman RD [Pubmed: 34531522] [DOI: 10.1038/s42003-021-02615-y] |
381.2 GB | — |
| 2025-02-10 |  |
Vibrio cholerae DdmD apo complex [multiple data sets in TIFF and MRC formats] | Luuk L, Jinek M [Pubmed: 38870273] [DOI: 10.1126/science.adq0534] |
1.5 TB | 2.55 Å |
| 2025-02-10 |  |
Vibrio cholerae DdmD-DdmE holo complex [multiple data sets in TIFF and MRC formats] | Luuk L, Jinek M [Pubmed: 38870273] [DOI: 10.1126/science.adq0534] |
1.6 TB | 2.56 Å |
| 2015-02-26 |  |
VipA/VipB, sheath of the bacterial type IV secretion system, micrographs for helical reconstruction taken on a K2 detector [77 micrographs in MRC format] | Kudryashev M, Wang R, Brackmann M, Scherer S, Maier T, DiMaio F, Baker D, Stahlberg H, Egelman EH, Basler M [Pubmed: 25723169] [DOI: 10.1016/j.cell.2015.01.037] |
4.1 GB | 3.5 Å |
| 2026-09-24 |  |
Viral chemokine receptor US28 bound to engineered fractalkine and human Gq [11773 multi-frame micrographs composed of 50 frames each in TIFF format] | Tsutsumi N [DOI: 10.1038/s41467-026-78122-7] |
5.4 TB | 2.58 - 2.89 Å |
| 2026-08-25 |  |
Visualization of membrane-stabilized SorCS2 cis interactions [multiple data sets in MRC format] | Beugelink JW, Janssen BJC [Pubmed: 42568954] [DOI: 10.1016/j.yjsbx.2026.100155] |
2.9 GB | 9.6 - 31.0 Å |
| 2016-02-04 |  |
Volta phase plate cryo-EM of the small protein complex Prx3 [multiple data sets in MRC and Other formats] | Khoshouei MK [Pubmed: 26817416] [DOI: 10.1038/ncomms10534] |
612.5 GB | 4.4 Å |
| 2018-01-31 |  |
Volta phase plate data collection facilitates image processing and cryo-EM structure determination [219 multi-frame micrographs composed of 30 frames each in TIFF format] | von Loeffelholz O, Klaholz BP [Pubmed: 29337113] [DOI: 10.1016/j.jsb.2018.01.003] |
45.9 GB | 4.6 Å |
| 2018-01-25 |  |
Volta phase plate data collection facilitates image processing and cryo-EM structure determination [318 multi-frame micrographs composed of 30 frames each in TIFF format] | von Loeffelholz O, Klaholz BP [Pubmed: 29337113] [DOI: 10.1016/j.jsb.2018.01.003] |
64.0 GB | 3.9 Å |
| 2018-01-26 |  |
Volta phase plate data collection facilitates image processing and cryo-EM structure determination [435 multi-frame micrographs composed of 30 frames each in TIFF format] | von Loeffelholz O, Klaholz BP [Pubmed: 29337113] [DOI: 10.1016/j.jsb.2018.01.003] |
89.4 GB | 4.4 Å |
| 2016-03-16 |  |
Volta phase plate in-focus dataset of T20S proteasome [158 multi-frame micrographs composed of 12 frames each in MRC format] | Danev R, Baumeister W [Pubmed: 26949259] [DOI: 10.7554/eLife.13046] |
50.3 GB | 3.2 Å |
| 2016-11-10 |  |
Volta phase plate with defocus cryo-EM dataset of Thermoplasma acidophilum 20S proteasome [427 multi-frame micrographs composed of 24 frames each in TIFF format] | Danev R, Tegunov D, Baumeister W [Pubmed: 28109158] [DOI: 10.1101/085530] |
92.5 GB | 2.2 - 2.4 Å |
